Limba
Romanian
Romanian
English|
ELK6636-96T, Human WNT11(Wingless Type MMTV Integration Site Family, Member 11) ELISA Kit, 96T
2.963,10 RON
Human WNT11(Wingless Type MMTV Integration Site Family, Member 11) ELISA Kit
SKU
ELK6636-96T
Alternative Names: wnt-11; wnt11; WNT11; wnt11b
Species: Human
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT11 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: wnt-11; wnt11; WNT11; wnt11b Species: Human Assay Type: Sandwich Sensitivity: 0.055 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Metabolic pathway;Bone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT11 in the samples is then determined by comparing the OD of the samples to the standard curve. |
