ELK6573-48T, Mouse PRDX4(Peroxiredoxin 4) ELISA Kit, 48T

ELK6573-48T, Mouse PRDX4(Peroxiredoxin 4) ELISA Kit, 48T

ELK6574-48T, Mouse RAD50(DNA Repair Protein RAD50) ELISA Kit, 48T

ELK6574-48T, Mouse RAD50(DNA Repair Protein RAD50) ELISA Kit, 48T

ELK6573-96T, Mouse PRDX4(Peroxiredoxin 4) ELISA Kit, 96T

2.963,10 RON

Mouse PRDX4(Peroxiredoxin 4) ELISA Kit

SKU
ELK6573-96T

Alternative Names: Antioxidant enzyme AOE372; Thioredoxin-dependent peroxide reductase A0372

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRDX4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRDX4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRDX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRDX4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Antioxidant enzyme AOE372; Thioredoxin-dependent peroxide reductase A0372

Species: Mouse

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRDX4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRDX4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRDX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRDX4 in the samples is then determined by comparing the OD of the samples to the standard curve.