ELK6565-96T, Human SEMA3E(Semaphorin 3E) ELISA Kit, 96T

ELK6565-96T, Human SEMA3E(Semaphorin 3E) ELISA Kit, 96T

ELK6566-96T, Mouse KLF1(Kruppel Like Factor 1, Erythroid) ELISA Kit, 96T

ELK6566-96T, Mouse KLF1(Kruppel Like Factor 1, Erythroid) ELISA Kit, 96T

ELK6566-48T, Mouse KLF1(Kruppel Like Factor 1, Erythroid) ELISA Kit, 48T

2.142,00 RON

Mouse KLF1(Kruppel Like Factor 1, Erythroid) ELISA Kit

SKU
ELK6566-48T

Alternative Names: EKLF; Erythroid krueppel-like transcription factor

Species: Mouse

Assay Type: Sandwich

Sensitivity: 5.8 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KLF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KLF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: EKLF; Erythroid krueppel-like transcription factor

Species: Mouse

Assay Type: Sandwich

Sensitivity: 5.8 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KLF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KLF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLF1 in the samples is then determined by comparing the OD of the samples to the standard curve.