ELK6511-96T, Rat ATP6AP2(ATPase, H+ Transporting, Lysosomal Accessory Protein 2) ELISA Kit, 96T

Alternative Names: ATP6IP2; CAPER; ELDF10; XMRE; Renin/prorenin receptor; Embryonic liver differentiation factor 10; Vacuolar ATP synthase membrane sector-associated protein M8-9

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATP6AP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATP6AP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATP6AP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATP6AP2 in the samples is then determined by comparing the OD of the samples to the standard curve.