ELK6385-48T, Mouse LOXL1(Lysyl Oxidase Like Protein 1) ELISA Kit, 48T

ELK6385-48T, Mouse LOXL1(Lysyl Oxidase Like Protein 1) ELISA Kit, 48T

ELK6386-48T, Rat F7(Coagulation Factor VII) ELISA Kit, 48T

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ELK6385-96T, Mouse LOXL1(Lysyl Oxidase Like Protein 1) ELISA Kit, 96T

2.963,10 RON

Mouse LOXL1(Lysyl Oxidase Like Protein 1) ELISA Kit

SKU
ELK6385-96T

Alternative Names: LOL; LOXL; Lysyl oxidase homolog 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LOXL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LOXL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LOXL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LOXL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: LOL; LOXL; Lysyl oxidase homolog 1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 31 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LOXL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LOXL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LOXL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LOXL1 in the samples is then determined by comparing the OD of the samples to the standard curve.