Rat SCGN(Secretagogin) ELISA Kit
Alternative Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 24.7 pg/mL
Standard: 5000 pg/mL
Detection range: 78.13-5000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2h
Research Area: Metabolic pathway;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein Species: Rat Assay Type: Competitive Inhibition Sensitivity: 24.7 pg/mL Standard: 5000 pg/mL Detection range: 78.13-5000 pg/mL Sample type: Serum, plasma and other biological fluids. Assay length: 2h Research Area: Metabolic pathway; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve. |