ELK6364-48T, Rat SCGN(Secretagogin) ELISA Kit, 48T

Alternative Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 24.7 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Metabolic pathway;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve.