ELK6361-48T, Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit, 48T

ELK6361-48T, Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit, 48T

ELK6362-48T, Human BCL2L11(Bcl2 Like Protein 11) ELISA Kit, 48T

ELK6362-48T, Human BCL2L11(Bcl2 Like Protein 11) ELISA Kit, 48T

ELK6361-96T, Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit, 96T

2.963,10 RON

Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit

SKU
ELK6361-96T

Alternative Names: GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GCLC in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GCLC in the samples is then determined by comparing the OD of the samples to the standard curve.