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ELK6346-48T, Rat MIA1(Melanoma Inhibitory Activity Protein 1) ELISA Kit, 48T
2.142,00 RON
Rat MIA1(Melanoma Inhibitory Activity Protein 1) ELISA Kit
SKU
ELK6346-48T
Alternative Names: CD-RAP; Melanoma-derived growth regulatory protein
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CD-RAP; Melanoma-derived growth regulatory protein Species: Rat Assay Type: Sandwich Sensitivity: 0.57 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIA1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
