Limba
Romanian
Romanian
English|
ELK6274-96T, Rat EDN2(Endothelin 2) ELISA Kit, 96T
2.963,10 RON
Rat EDN2(Endothelin 2) ELISA Kit
SKU
ELK6274-96T
Alternative Names: ET2; PPET2; Preproendothelin-2
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 2.67 pg/mL
Standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 2h
Research Area: Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ET2; PPET2; Preproendothelin-2 Species: Rat Assay Type: Competitive Inhibition Sensitivity: 2.67 pg/mL Standard: 500 pg/mL Detection range: 7.82-500 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay length: 2h Research Area: Cardiovascular biology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
