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ELK6244-96T, Rat PPOX(Protoporphyrinogen Oxidase) ELISA Kit, 96T
2.963,10 RON
Rat PPOX(Protoporphyrinogen Oxidase) ELISA Kit
SKU
ELK6244-96T
Alternative Names: VP; PPO; V290M; Variegate Porphyria
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.052 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPOX in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: VP; PPO; V290M; Variegate Porphyria Species: Rat Assay Type: Sandwich Sensitivity: 0.052 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PPOX in the samples is then determined by comparing the OD of the samples to the standard curve. |
