ELK6149-48T, Mouse ACP2(Acid Phosphatase 2, Lysosomal) ELISA Kit, 48T

ELK6149-48T, Mouse ACP2(Acid Phosphatase 2, Lysosomal) ELISA Kit, 48T

ELK6150-48T, Rat DLG4(Discs, Large Homolog 4) ELISA Kit, 48T

ELK6150-48T, Rat DLG4(Discs, Large Homolog 4) ELISA Kit, 48T

ELK6149-96T, Mouse ACP2(Acid Phosphatase 2, Lysosomal) ELISA Kit, 96T

2.963,10 RON

Mouse ACP2(Acid Phosphatase 2, Lysosomal) ELISA Kit

SKU
ELK6149-96T

Alternative Names: Lysosomal Acid Phosphatase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Lysosomal Acid Phosphatase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACP2 in the samples is then determined by comparing the OD of the samples to the standard curve.