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ELK6140-96T, Dog ENG(EnDoglin) ELISA Kit, 96T
3.736,60 RON
Dog ENG(EnDoglin) ELISA Kit
SKU
ELK6140-96T
Alternative Names: CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1
Species: Dog
Assay Type: Sandwich
Sensitivity: 0.3 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ENG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ENG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ENG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ENG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1 Species: Dog Assay Type: Sandwich Sensitivity: 0.3 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 3.5h Research Area: Tumor immunity;Infection immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ENG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ENG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ENG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ENG in the samples is then determined by comparing the OD of the samples to the standard curve. |
