ELK6055-96T, Mouse ARPC4(Actin Related Protein 2/3 Complex Subunit 4) ELISA Kit, 96T

ELK6055-96T, Mouse ARPC4(Actin Related Protein 2/3 Complex Subunit 4) ELISA Kit, 96T

ELK6056-96T, Mouse KL(Klotho) ELISA Kit, 96T

ELK6056-96T, Mouse KL(Klotho) ELISA Kit, 96T

ELK6056-48T, Mouse KL(Klotho) ELISA Kit, 48T

2.142,00 RON

Mouse KL(Klotho) ELISA Kit

SKU
ELK6056-48T

Alternative Names: Kl ; KLOT; Klotho peptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.25 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KL in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Kl ; KLOT; Klotho peptide

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.25 pg/mL

Standard: 200 pg/mL

Detection range: 3.13-200 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KL in the samples is then determined by comparing the OD of the samples to the standard curve.