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ELK6044-96T, Dog I-PTH(IntactParathormone) ELISA Kit, 96T
3.736,60 RON
Dog I-PTH(IntactParathormone) ELISA Kit
SKU
ELK6044-96T
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Species: Dog
Assay Type: Sandwich
Sensitivity: 4.15 pg/mL
Standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone Species: Dog Assay Type: Sandwich Sensitivity: 4.15 pg/mL Standard: 800 pg/mL Detection range: 12.5-800 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Endocrinology;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve. |
