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ELK6030-96T, Dog C3a(Complement Component 3a) ELISA Kit, 96T
3.736,60 RON
Dog C3a(Complement Component 3a) ELISA Kit
SKU
ELK6030-96T
Alternative Names: Complement Component 3a
Species: Dog
Assay Type: Sandwich
Sensitivity: 1.29 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum and plasma
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Complement Component 3a Species: Dog Assay Type: Sandwich Sensitivity: 1.29 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum and plasma Assay length: 3.5h Research Area: Infection immunity;Immune molecule;Autoimmunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog C3a in the samples is then determined by comparing the OD of the samples to the standard curve. |
