ELK5992-96T, Dog HMGB-1(High Mobility Group Protein B1) ELISA Kit, 96T

Alternative Names: HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1

Species: Dog

Assay Type: Sandwich

Sensitivity: 2.73 pg/mL

Standard: 400 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.