ELK5973-48T, Dog SELE(Selectin, Endothelium) ELISA Kit, 48T

Alternative Names: CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E

Species: Dog

Assay Type: Sandwich

Sensitivity: 2.84 pg/mL

Standard: 400 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SELE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SELE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SELE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SELE in the samples is then determined by comparing the OD of the samples to the standard curve.