ELK5930-48T, Horse LEP(Leptin) ELISA Kit, 48T

Alternative Names: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Species: Horse

Assay Type: Sandwich

Sensitivity: 0.256 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse LEP in the samples is then determined by comparing the OD of the samples to the standard curve.