ELK5919-48T, Pig AP(Aprotinin) ELISA Kit, 48T

Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

Species: Pig

Assay Type: Competitive Inhibition

Sensitivity: 0.47 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 2h

Research Area: Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve.