Pig AP(Aprotinin) ELISA Kit
Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 0.47 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2h
Research Area: Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.260,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor Species: Pig Assay Type: Competitive Inhibition Sensitivity: 0.47 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma and other biological fluids. Assay length: 2h Research Area: Enzyme & Kinase;Cardiovascular biology;Human gene deletion;Hepatology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig AP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig AP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig AP in the samples is then determined by comparing the OD of the samples to the standard curve. |