ELK5894-96T, Cattle VF(Visfatin) ELISA Kit, 96T

Alternative Names: Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

Species: Cattle

Assay Type: Sandwich

Sensitivity: 1.27 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VF in the samples is then determined by comparing the OD of the samples to the standard curve.