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ELK5763-96T, Rat ALOX5(Arachidonate-5-Lipoxygenase) ELISA Kit, 96T
2.963,10 RON
Rat ALOX5(Arachidonate-5-Lipoxygenase) ELISA Kit
SKU
ELK5763-96T
Alternative Names: LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.27 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALOX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALOX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALOX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALOX5 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: LOX5; 5-LOX; ALOX-5; 5-LO; 5LPG; LOG5; Lipoxygenase 5 Species: Rat Assay Type: Sandwich Sensitivity: 0.27 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALOX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALOX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALOX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALOX5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
