ELK5742-48T, Rat PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit, 48T

Alternative Names: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide

Species: Rat

Assay Type: Sandwich

Sensitivity: 11.5 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Hepatology;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.