ELK5732-96T, Dog OPN(Osteopontin) ELISA Kit, 96T

Alternative Names: SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

Species: Dog

Assay Type: Sandwich

Sensitivity: 0.134 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog OPN in the samples is then determined by comparing the OD of the samples to the standard curve.