Pig TM(Thrombomodulin) ELISA Kit
Alternative Names: CD141; THBD; BDCA3; THRM; Fetomodulin
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.29 ng/mL
Standard: 50 ng/mL
Detection range: 0.79-50 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Metabolic pathway;Hematology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TM in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.260,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: CD141; THBD; BDCA3; THRM; Fetomodulin Species: Pig Assay Type: Sandwich Sensitivity: 0.29 ng/mL Standard: 50 ng/mL Detection range: 0.79-50 ng/mL Sample type: plasma Assay length: 3.5h Research Area: Metabolic pathway;Hematology;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig TM in the samples is then determined by comparing the OD of the samples to the standard curve. |