Limba
Romanian
Romanian
English|
ELK5661-96T, Dog IFNg(Interferon Gamma) ELISA Kit, 96T
3.736,60 RON
Dog IFNg(Interferon Gamma) ELISA Kit
SKU
ELK5661-96T
Alternative Names: IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Species: Dog
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IFNg in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon Species: Dog Assay Type: Sandwich Sensitivity: 5.9 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Cytokine;Infection immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IFNg in the samples is then determined by comparing the OD of the samples to the standard curve. |
