ELK5652-48T, Dog SPA(Surfactant Associated Protein A) ELISA Kit, 48T

Alternative Names: PRL; SFTPA; PSAP; PSPA; SFTP; SFTPA1; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein

Species: Dog

Assay Type: Sandwich

Sensitivity: 2.64 pg/mL

Standard: 400 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, lung lavage fluid, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPA in the samples is then determined by comparing the OD of the samples to the standard curve.