Limba
Romanian
Romanian
English|
ELK5637-96T, Rat DEFb1(Defensin Beta 1) ELISA Kit, 96T
2.963,10 RON
Rat DEFb1(Defensin Beta 1) ELISA Kit
SKU
ELK5637-96T
Alternative Names: B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1
Species: Rat
Assay Type: Sandwich
Sensitivity: 19.27 pg/mL
Standard: 3000 pg/mL
Detection range: 46.88-3000 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DEFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DEFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DEFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DEFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1 Species: Rat Assay Type: Sandwich Sensitivity: 19.27 pg/mL Standard: 3000 pg/mL Detection range: 46.88-3000 pg/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Infection immunity;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DEFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DEFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DEFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DEFb1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
