ELK5532-48T, Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit, 48T

ELK5532-48T, Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit, 48T

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ELK5532-96T, Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit, 96T

2.963,10 RON

Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit

SKU
ELK5532-96T

Alternative Names: P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 1.49 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Infection immunity;Bone metabolism;Rheumatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIICP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 1.49 ng/mL

Standard: 300 ng/mL

Detection range: 4.69-300 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Infection immunity;Bone metabolism;Rheumatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PIICP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIICP in the samples is then determined by comparing the OD of the samples to the standard curve.