ELK5472-96T, Human PNPLA2(Patatin Like Phospholipase Domain Containing Protein 2) ELISA Kit, 96T

Alternative Names: ATGL; PEDF-R; TTS2; Desnutrin; Pigment epithelium-derived factor; Transport-secretion protein 2; Adipose Triglyceride Lipase; Calcium-independent phospholipase A2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.