ELK5406-96T, Human PI(Proinsulin) ELISA Kit, 96T

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ELK5407-48T, Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit, 48T

2.142,00 RON

Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit

SKU
ELK5407-48T

Alternative Names: OL

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.52 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OxLDL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OxLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OxLDL in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: OL

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.52 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OxLDL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OxLDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OxLDL in the samples is then determined by comparing the OD of the samples to the standard curve.