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ELK5401-48T, Human ES(Endostatin) ELISA Kit, 48T
2.142,00 RON
Human ES(Endostatin) ELISA Kit
SKU
ELK5401-48T
Alternative Names: FLJ27325; FLJ34914; KNO; KNO1; MGC74745;
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 1.74 ng/mL
Standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Tumor immunity;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ES protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ES in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: FLJ27325; FLJ34914; KNO; KNO1; MGC74745; Species: Human Assay Type: Competitive Inhibition Sensitivity: 1.74 ng/mL Standard: 400 ng/mL Detection range: 6.25-400 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 2h Research Area: Tumor immunity;Cardiovascular biology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ES protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ES in the samples is then determined by comparing the OD of the samples to the standard curve. |
