ELK5381-48T, Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit, 48T

ELK5381-48T, Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit, 48T

ELK5382-48T, Mouse ACVA(Activin A) ELISA Kit, 48T

ELK5382-48T, Mouse ACVA(Activin A) ELISA Kit, 48T

ELK5381-96T, Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit, 96T

2.963,10 RON

Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit

SKU
ELK5381-96T

Alternative Names: LH-B; CGB4; LSH-B; hLHB; Lutropin Subunit Beta; Interstitial Cell Stimulating Hormone,Beta Chain

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 89.2 pg/mL

Standard: 20000 pg/mL

Detection range: 312.5-20000 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Reproductive science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LHb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LHb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LHb in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: LH-B; CGB4; LSH-B; hLHB; Lutropin Subunit Beta; Interstitial Cell Stimulating Hormone,Beta Chain

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 89.2 pg/mL

Standard: 20000 pg/mL

Detection range: 312.5-20000 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 2h

Research Area: Reproductive science;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LHb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LHb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LHb in the samples is then determined by comparing the OD of the samples to the standard curve.