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ELK5359-96T, Human 20S-PSM(20S-Proteasome) ELISA Kit, 96T
2.963,10 RON
Human 20S-PSM(20S-Proteasome) ELISA Kit
SKU
ELK5359-96T
Alternative Names: CP; 20SPSM; 20S Core Particle
Species: Human
Assay Type: Sandwich
Sensitivity: 0.66 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CP; 20SPSM; 20S Core Particle Species: Human Assay Type: Sandwich Sensitivity: 0.66 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Tissue homogenates, cell lysates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 20S-PSM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve. |
