ELK5334-48T, Human TrxR2(Thioredoxin Reductase 2) ELISA Kit, 48T

ELK5334-48T, Human TrxR2(Thioredoxin Reductase 2) ELISA Kit, 48T

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ELK5334-96T, Human TrxR2(Thioredoxin Reductase 2) ELISA Kit, 96T

2.963,10 RON

Human TrxR2(Thioredoxin Reductase 2) ELISA Kit

SKU
ELK5334-96T

Alternative Names: TR; TXNRD2; TR3; SelZ; Thioredoxin Reductase Beta; Selenoprotein Z

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrxR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrxR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrxR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrxR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: TR; TXNRD2; TR3; SelZ; Thioredoxin Reductase Beta; Selenoprotein Z

Species: Human

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TrxR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TrxR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TrxR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TrxR2 in the samples is then determined by comparing the OD of the samples to the standard curve.