ELK5269-96T, Human Smad1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit, 96T

ELK5269-96T, Human Smad1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit, 96T

ELK5270-96T, Rabbit IFNb(Interferon Beta) ELISA Kit, 96T

ELK5270-96T, Rabbit IFNb(Interferon Beta) ELISA Kit, 96T

ELK5270-48T, Rabbit IFNb(Interferon Beta) ELISA Kit, 48T

2.689,40 RON

Rabbit IFNb(Interferon Beta) ELISA Kit

SKU
ELK5270-48T

Alternative Names: IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 5.6 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 5.6 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IFNb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IFNb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IFNb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IFNb in the samples is then determined by comparing the OD of the samples to the standard curve.