ELK5235-48T, Human HRSP12(Heat Responsive Protein 12) ELISA Kit, 48T

ELK5235-48T, Human HRSP12(Heat Responsive Protein 12) ELISA Kit, 48T

ELK5236-48T, Rat FASL(Factor Related Apoptosis Ligand) ELISA Kit, 48T

ELK5236-48T, Rat FASL(Factor Related Apoptosis Ligand) ELISA Kit, 48T

ELK5235-96T, Human HRSP12(Heat Responsive Protein 12) ELISA Kit, 96T

2.963,10 RON

Human HRSP12(Heat Responsive Protein 12) ELISA Kit

SKU
ELK5235-96T

Alternative Names: PSP; P14.5; UK114; Ribonuclease UK114; 14.5 kDa translational inhibitor protein; Heat-responsive protein 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HRSP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HRSP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HRSP12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HRSP12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mai multe informatii
Price 2.490,00 RON (preturile sunt fara TVA)
Description

Alternative Names: PSP; P14.5; UK114; Ribonuclease UK114; 14.5 kDa translational inhibitor protein; Heat-responsive protein 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HRSP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HRSP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HRSP12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HRSP12 in the samples is then determined by comparing the OD of the samples to the standard curve.