ELK5175-48T, Human POLd(Polymerase DNA Directed Delta 1) ELISA Kit, 48T

ELK5175-48T, Human POLd(Polymerase DNA Directed Delta 1) ELISA Kit, 48T

ELK5176-48T, Human WARS(Tryptophanyl tRNA Synthetase) ELISA Kit, 48T

ELK5176-48T, Human WARS(Tryptophanyl tRNA Synthetase) ELISA Kit, 48T

ELK5175-96T, Human POLd(Polymerase DNA Directed Delta 1) ELISA Kit, 96T

2.963,10 RON

Human POLd(Polymerase DNA Directed Delta 1) ELISA Kit

SKU
ELK5175-96T

Alternative Names: CDC2; POLD1; DNA polymerase subunit delta p125

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human POLd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POLd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human POLd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POLd in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CDC2; POLD1; DNA polymerase subunit delta p125

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human POLd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POLd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human POLd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POLd in the samples is then determined by comparing the OD of the samples to the standard curve.