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ELK5156-96T, Human OGT(O-Linked-N-Acetylglucosamine Transferase) ELISA Kit, 96T
2.963,10 RON
Human OGT(O-Linked-N-Acetylglucosamine Transferase) ELISA Kit
SKU
ELK5156-96T
Alternative Names: HRNT1; O-GLCNAC; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110
Species: Human
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGT in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HRNT1; O-GLCNAC; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc transferase subunit p110 Species: Human Assay Type: Sandwich Sensitivity: 0.59 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Tissue homogenates, cell lysates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OGT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGT in the samples is then determined by comparing the OD of the samples to the standard curve. |
