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ELK5123-48T, Human Hepc(Hepcidin) ELISA Kit, 48T
2.142,00 RON
Human Hepc(Hepcidin) ELISA Kit
SKU
ELK5123-48T
Alternative Names: HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.218 ng/mL
Standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 2h
Research Area: Metabolic pathway;Endocrinology;Hematology;Genetic science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Hepc protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Hepc in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor Species: Human Assay Type: Competitive Inhibition Sensitivity: 0.218 ng/mL Standard: 40 ng/mL Detection range: 0.63-40 ng/mL Sample type: Tissue homogenates and other biological fluids. Assay length: 2h Research Area: Metabolic pathway;Endocrinology;Hematology;Genetic science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Hepc protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Hepc. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Hepc in the samples is then determined by comparing the OD of the samples to the standard curve. |
