ELK5104-48T, Human CD8(Cluster ofDifferentiation 8) ELISA Kit, 48T

Alternative Names: p32; CD8-A; Leu2; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; Cluster Of Differentiation 8a

Species: Human

Assay Type: Sandwich

Sensitivity: 0.64 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD8 in the samples is then determined by comparing the OD of the samples to the standard curve.