ELK5083-96T, Human ECE2(Endothelin Converting Enzyme 2) ELISA Kit, 96T

Alternative Names: Methyltransferase-like region

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 45.9 pg/mL

Standard: 10000 pg/mL

Detection range: 156.25-10000 pg/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 2h

Research Area: Enzyme & Kinase;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ECE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ECE2 in the samples is then determined by comparing the OD of the samples to the standard curve.