ELK5049-96T, Human TYROBP(TYRO Protein Tyrosine Kinase Binding Protein) ELISA Kit, 96T

Alternative Names: DAP12; KARAP; PLOSL; Killer Activating Receptor Associated Protein; DNAX-Activation Protein 12

Species: Human

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TYROBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TYROBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TYROBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TYROBP in the samples is then determined by comparing the OD of the samples to the standard curve.