ELK4979-96T, Human SESN2(Sestrin 2) ELISA Kit, 96T

ELK4979-96T, Human SESN2(Sestrin 2) ELISA Kit, 96T

ELK4980-96T, Human SPP2(Secreted Phosphoprotein 2) ELISA Kit, 96T

ELK4980-96T, Human SPP2(Secreted Phosphoprotein 2) ELISA Kit, 96T

ELK4980-48T, Human SPP2(Secreted Phosphoprotein 2) ELISA Kit, 48T

2.142,00 RON

Human SPP2(Secreted Phosphoprotein 2) ELISA Kit

SKU
ELK4980-48T

Alternative Names: SPP24; Secreted phosphoprotein 24

Species: Human

Assay Type: Sandwich

Sensitivity: 28.4 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SPP24; Secreted phosphoprotein 24

Species: Human

Assay Type: Sandwich

Sensitivity: 28.4 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPP2 in the samples is then determined by comparing the OD of the samples to the standard curve.