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ELK4951-96T, Human C4a(Complement Component 4a) ELISA Kit, 96T
2.963,10 RON
Human C4a(Complement Component 4a) ELISA Kit
SKU
ELK4951-96T
Alternative Names: C4a anaphylatoxin
Species: Human
Assay Type: Sandwich
Sensitivity: 0.151 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4a in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: C4a anaphylatoxin Species: Human Assay Type: Sandwich Sensitivity: 0.151 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Immune molecule;Endocrinology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4a in the samples is then determined by comparing the OD of the samples to the standard curve. |
