ELK4934-48T, Human UQCR(Ubiquinol Cytochrome C Reductase) ELISA Kit, 48T

ELK4934-48T, Human UQCR(Ubiquinol Cytochrome C Reductase) ELISA Kit, 48T

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ELK4934-96T, Human UQCR(Ubiquinol Cytochrome C Reductase) ELISA Kit, 96T

2.963,10 RON

Human UQCR(Ubiquinol Cytochrome C Reductase) ELISA Kit

SKU
ELK4934-96T

Alternative Names: QCR10; Cytochrome b-c1 Complex Subunit 10; Complex III subunit XI; Ubiquinol-cytochrome c reductase complex 6.4 kDa protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UQCR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UQCR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UQCR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UQCR in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: QCR10; Cytochrome b-c1 Complex Subunit 10; Complex III subunit XI; Ubiquinol-cytochrome c reductase complex 6.4 kDa protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.27 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UQCR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UQCR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UQCR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UQCR in the samples is then determined by comparing the OD of the samples to the standard curve.