ELK4888-48T, Human KLK1(Kallikrein 1) ELISA Kit, 48T

ELK4888-48T, Human KLK1(Kallikrein 1) ELISA Kit, 48T

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ELK4888-96T, Human KLK1(Kallikrein 1) ELISA Kit, 96T

2.963,10 RON

Human KLK1(Kallikrein 1) ELISA Kit

SKU
ELK4888-96T

Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 27.51 pg/mL

Standard: 6000 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Hepatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 27.51 pg/mL

Standard: 6000 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Enzyme & Kinase;Tumor immunity;Hepatology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.