ELK4881-48T, Rat BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit, 48T

Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.134 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Kidney biomarker;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.