Limba
Romanian
Romanian
English|
ELK4864-96T, Rat NES1(Nesfatin 1) ELISA Kit, 96T
2.963,10 RON
Rat NES1(Nesfatin 1) ELISA Kit
SKU
ELK4864-96T
Alternative Names: Nesfatin-1
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 182.5 pg/mL
Standard: 40000 pg/mL
Detection range: 625-40000 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2h
Research Area: Glycolipid metabolism
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Nesfatin-1 Species: Rat Assay Type: Competitive Inhibition Sensitivity: 182.5 pg/mL Standard: 40000 pg/mL Detection range: 625-40000 pg/mL Sample type: Serum, plasma and other biological fluids Assay length: 2h Research Area: Glycolipid metabolism Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NES1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
