ELK4859-96T, Human IGFIIE(Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit, 96T

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ELK4860-96T, Human ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit, 96T

ELK4860-96T, Human ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit, 96T

ELK4860-48T, Human ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit, 48T

2.142,00 RON

Human ADCYAP1(Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit

SKU
ELK4860-48T

Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.55 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ADCYAP1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADCYAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.55 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ADCYAP1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADCYAP1 in the samples is then determined by comparing the OD of the samples to the standard curve.