ELK4853-48T, Human aCTx(Alpha-Crosslaps) ELISA Kit, 48T

Alternative Names: Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 25.3 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 2h

Research Area: Bone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.